1. What is the significance of the 'cry' gene in biotechnology?
2. What is the main goal of biotechnological applications in food production and health?
3. Which technique is used to transfer DNA fragments from agarose gel to a nitrocellulose membrane?
4. What is a key benefit of using host plant-generated dsRNA in pest control?
5. What is the outcome of RNAi in nematodes feeding on transgenic plants?
6. What is the purpose of a nutrient medium in tissue culture?
7. What is the primary role of microbes in biotechnology?
8. What is the main purpose of using transgenic mice in vaccine testing?
9. Which of the following is NOT a feature of pBR322?
10. What is the role of competent cells in transformation?
11. What is the effect of Bt toxin on non-target organisms?
12. What ethical issue is associated with biotechnology?
13. What ethical standard is emphasized in the text regarding genetic modification?
14. Why is enzyme engineering important in biotechnology?
15. What is the role of beta-galactosidase in blue-white screening?
16. How many recombinant therapeutics are currently marketed in India?
17. What is a transgenic animal?
18. What does 'totipotency' refer to in plant tissue culture?
19. What is the function of agarose in gel electrophoresis?
20. Which of the following is a disadvantage of using antibiotic resistance genes as selectable markers?